Optimization of Unnatural Base Pair Packing for Polymerase Recognition
نویسندگان
چکیده
منابع مشابه
Highly specific unnatural base pair systems as a third base pair for PCR amplification
Toward the expansion of the genetic alphabet of DNA, we present highly efficient unnatural base pair systems as an artificial third base pair for PCR. Hydrophobic unnatural base pair systems between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) were fine-tuned for efficient PCR, by assessing the amplification efficiency and fidelity using different polymerases and ...
متن کاملSelective transcription of an unnatural naphthyridine:imidazopyridopyrimidine base pair containing four hydrogen bonds with T7 RNA polymerase.
The naphthyridine:imidazopyridopyrimidine base pair is the first base pair containing four hydrogen bonds that can be replicated selectively and efficiently by the use of DNA polymerases. Herein we describe the synthesis of naphthyridine-C-ribonucleoside 5'-triphosphate (rNaTP) and transcription reactions catalyzed by T7 RNA polymerase with rNaTP and template DNA containing imidazopyridopyrimid...
متن کاملExploiting the enzymatic recognition of an unnatural base pair to develop a universal genetic analysis system.
BACKGROUND With the invention of the DNA chip, genome-wide analysis is now a reality. Unfortunately, solid-phase detection systems such as the DNA chip suffer from a narrow range in quantification and sensitivity. Today the best methodology for sensitive, wide dynamic range quantification and genotyping of nucleic acids is real-time PCR. However, multiplexed real-time PCR technologies require c...
متن کاملSubstituent effects on the pairing and polymerase recognition of simple unnatural base pairs
As part of an effort to develop stable and replicable unnatural base pairs, we have evaluated a large number of unnatural nucleotides with predominantly hydrophobic nucleobases. Despite its limited aromatic surface area, a nucleobase analog scaffold that has emerged as being especially promising is the simple phenyl ring. Modifications of this scaffold with methyl and fluoro groups have been sh...
متن کاملAN UNNATURAL BAsE pAiR sYsTEM FOR ThE ExpANsiON OF GENETic iNFORMATiON
Expansion of the genetic alphabet by unnatural base pair systems is an attractive future biotechnology.1 DNA fragments containing unnatural base pairs function as templates for the site-specific incorporation of extra components into nucleic acids and proteins by biosyntheses, according to the Central Dogma of molecular biology replication, transcription, and translation. Nucleic acids and prot...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2006
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja057575m